30 million LCL1 cells were crosslinked with 1% formaldehyde and harvested for sonication. Then cell lysates including sheared genomic DNA were immunoprecipitated with the indicated antibody (anti-E2F1, anti-E2F6, anti-Rb, anti-HDAC1, and anti-HDAC2) or normal IgG to collect the DNA fragments that can bind with the interest proteins. The immune complexes were incubated with the salmon sperm DNA/protein A agarose slurry, and the collected DNAs were purified for further processes. The DNA library was prepared with TruSeq ChIP Library Preparation Kit (IP-202-1012; Illumina, San Diego, CA). Then the purified DNAs were sequenced using the Illumina HiSeq platform by the Genome Technology Access Center (GTAC) at the Washington University in St. Louis.